Lab Protocols:
DNA/RNA work:
Genomic DNA Preparation from RNAlater™ Preserved Tissues Protocol
Coral sperm collection for genome sequencing protocol
DNA extraction from frozen coral sperm protocol
Coral RNA isolation protocol (for small prep’s in 1.5mL or 2mL tubes)
Coral RNA isolation (for large prep’s in 15mL Falcon tubes)
RNA extraction for coral larvae
Coral tissue blasting protocol
Coral spawning:
Protein work:
Environmental samples:
Sterivex water filtration protocol
Lipid in water, lipid in sediment, and filtration for nucleic acid recovery protocol by ICoMM
Other lab work protocols:
Probe construction for in-situ hybridizations protocol
Symbiodinium spp. genotyping protocol
Orbicella faveolata host genotyping
Medina Lab, Laboratory notebook procedures (Very important for all lab members!)
And, finally we are in the process of compiling a list of frequently asked questions on general lab procedures. This will be posted soon!
*Note Montastraea faveolata was re-classified as Orbicella faveolata. For more details refer to Budd et al. (2012) Taxonomic classification of the reef coral family Mussidae (Cnidaria: Anthozoa: Scleractinia).