MedinaLAB

Pre-proposal

The goal of this program is to generate pilot data for a collaborative consortium manuscript using coral ancient DNA to reconstruct coral holobionts of the past. By recovering information from both the coral host and its associated microorganisms, including algal symbionts, bacteria, archaea, fungi, and viruses, we aim to examine how the composition and biology of the holobiont changed through time and across periods of extreme thermal stress. We are especially interested in well-dated samples for which the timing of thermal stress is independently supported by geochemical evidence from the coral skeleton or associated environmental records.

Each submitting group may propose three timepoints, ideally including one pre-industrial baseline and two timepoints spanning documented thermal stress events. We seek three biological replicates from each timepoint, for a total of nine samples and nine sequencing libraries per group. Applicants should briefly describe the rationale for the selected timepoints, the evidence supporting the timing and severity of the stress events, and the availability and suitability of the proposed biological replicates.

Applicant Information

Full Name

First

Last

Institutional Email Address

Alternative Email Address

Career Stage

Project Information

Project Title

Sample Information

Sample Type

Examples: coral core, skeletal archive, museum specimen, historical collection, modern comparison material, associated environmental sample

Coral taxon / species, if known

Sample location / reef / region

Approximate sample age or date range

Number of samples proposed

Brief sample description and provenance

Please describe where the samples come from, who holds them, and any relevant collection history.

Evidence of thermal stress or environmental event, if applicable

Please describe any geochemical, historical, monitoring, or other evidence linking the samples to an environmental event.

Permits, permissions, and sample availability

Please indicate whether the samples are already available for destructive sampling, and whether any permits, MTAs, export/import permissions, or institutional approvals may be required.

Sample species, Career

Existing metadata or paired data

Examples: geochemistry, growth bands, X-rays/CT scans, temperature records, bleaching history, symbiont data, previous sequencing, ecological metadata

Preproposal Abstract

Preproposal abstract

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Please provide a brief abstract describing the scientific questionwhy these samples are well suited for coraDNA analysis, and what the proposed project would contribute to the field. You may also include an optional figure. Suggested length: 250–500 words.